Extracts of Cynomorium songaricum Protect SK-N-SH Human Neuroblastoma Cells against Staurosporine-induced Apoptosis Potentially through their Radical Scavenging Activity

In traditional Chinese medicine a number of herbs are used to alleviate the symptoms of aging, among them the stems of Cynomorium songaricum, Cynomoriaceae. This study evaluated the protective effect of different extracts of C. songaricum on staurosporine-induced apoptotic cell death in SK-N-SH neuroblastoma cells. Staurosporine (100 nM) reduced cell viability to about 55%. The ethyl acetate fraction of C. songaricum significantly attenuated staurosporine-induced cell death at concentrations of 100 and 10 mu g/mL. On the other hand, the dichloromethane as well as water fractions showed no protective effects. In order to further analyse the protective mode of action, the superoxide anion scavenging activity of two extracts was evaluated in a xanthine/xanthine oxidase system. In this system, the EtOAc extract showed a good scavenging activity (IC50 value 2.9 mu g/mL) without inhibition of xanthine oxidase. In conclusion, the results prove the neuroprotective activity of C. songaricum extracts in vitro, thus supporting its traditional use. Copyright (C) 2008 John Wiley & Sons, Ltd.