Determination of atropine in pharmaceutical preparations by liquid chromatography with fluorescence detection

被引：28
|
作者：
Takahashi, M
Nagashima, M
Shigeoka, S
Nishijima, M
Kamata, K
机构：
[1] Tokyo Metropol. Res. Lab. Pub. Hlth., 24-1, Hyakunincho, 3-chome, S.
来源：
JOURNAL OF CHROMATOGRAPHY A | 1997年 / 775卷 / 1-2期
关键词：
pharmaceutical analysis; derivatization; LC; atropine; alkaloids; 1-anthroylnitrile;
D O I：
10.1016/S0021-9673(97)00305-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method is described for the determination of atropine in pharmaceutical preparations by precolumn derivatization and high-performance liquid chromatography (HPLC). Atropine is derivatized by reacting with 1-anthroylnitrile (1-AN) in acetone. The 1-AN derivative of atropine is then used for quantitative HPLC analysis on a reversed-phase column with fluorescence detection. The calibration graph was linear over a sample concentration range from 0.05 to 1.0 mu g/ml; the limit of detection was reached at a sample concentration of 0.01 mu g/ml. The proposed method was satisfactorily applied to the determination of atropine in pharmaceutical preparations. (C) 1997 Elsevier Science B.V.
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页码：137 / 141
页数：5
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