Identification of Live and Dead Bacteria: A Raman Spectroscopic Study

被引：26

作者：

Li, Runze ^{[1
]}

Dhankhar, Dinesh ^{[1
]}

Chen, Jie ^{[2
]}

Krishnamoorthi, Arjun ^{[1
]}

Cesario, Thomas C. ^{[3
]}

Rentzepis, Peter M. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Elect & Comp Engn, College Stn, TX 77843 USA

[2] Shanghai Jiao Tong Univ, Collaborat Innovat Ctr IFSA CICIFSA, Ctr Ultrafast Sci & Technol, Key Lab Laser Plasmas,Minist Educ,Sch Phys & Astr, Shanghai 200240, Peoples R China

[3] Univ Calif Irvine, Sch Med, Irvine, CA 92697 USA

来源：

IEEE ACCESS | 2019年 / 7卷

关键词：

Bacteria inactivation; optical spectroscopy; principal component analysis; Raman scattering; UV RESONANCE RAMAN; ESCHERICHIA-COLI; INFRARED-SPECTROSCOPY; PROTEIN DAMAGE; CELL RESPONSE; SPECTRA; INACTIVATION; IRRADIATION; DNA; NANOPARTICLES;

D O I：

10.1109/ACCESS.2019.2899006

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

Raman spectroscopy has been used to identify bacterial strains, bacterial components, such as protein and DNA bases, and the ratio of live to dead bacteria before and after exposure to ultraviolet (UV) radiation. New vibrational bands and changes in their intensity as a function of UV irradiation time have been recorded by high resolution Raman spectroscopy which made it possible to determine the mechanism of the UV inactivation of Escherichia coli (E. coli), Serratia marcescens (S. marcescens), and Micrococcus luteus (M. luteus) bacteria in saline solutions. We have also employed a novel, new, handheld spectrometer capable of recording, in situ, within minutes, the absorption, fluorescence, synchronous fluorescence, and Raman spectra of bacteria and other biological species and large molecules.

引用

页码：23549 / 23559

页数：11

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