Probing Electron-Transfer Times in Photosynthetic Reaction Centers by Hole-Burning Spectroscopy

A brief discussion is presented of transient hole-burned (HB) spectra (and the information that they provide) obtained for isolated reaction centers (RCs) from wild-type (WT) Rhodobacter sphaeroides, RCs containing zinc-bacteriochlorophylls (Zn-BChls), and RCs of Photosystem II (PSII) from spinach and Chlamydomonas reinhardtii. The shape of the spectral density and the strength of electron-phonon coupling in bacterial RCs are discussed. We focus, however, on heterogeneity of isolated PS II RCs from spinach and, in particular, Chlamydomonas reinhardtii, site energies of active (electron acceptor) and inactive pheophytins, the nature of the primary electron donor(s), and the possibility of multiple charge-separation (CS) pathways in the isolated PSII RC. We conclude with comments on current efforts in HB spectroscopy in the area of photosynthesis and future directions in HB spectroscopy.