Mitochondrial Ca2+ handling is crucial for generation of rhythmical Ca2+ waves in vascular interstitial cells from rabbit portal vein

Vasomotion is the rhythmical changes in vascular tone of various blood vessels. It was proposed that in rabbit portal vein (RPV) the spontaneous contractile activity is driven by vascular interstitial cells (VICs), since RPV VICs generate rhythmical changes in intracellular Ca2+ concentration ([Ca2+](i)) associated with membrane depolarisation in these cells. In this work, using confocal imaging in Fluo-3 loaded RPV VICs we studied if generation of rhythmical [Ca2+](i) changes is affected when Ca2+ handling by mitochondria is compromised. We also visualised mitochondria in VICs using Mito Tracker Green fluorescent dye. Our results showed that freshly dispersed RPV VICs generated rhythmical [Ca2+](i) oscillations with a frequency of 0.2-0.01 Hz. Imaging of VICs stained with Mito Tracker Green revealed abundant mitochondria in these cells with a higher density of the organelles in sub-plasmalemmar region compared to the central region of the cell. Oligomycin, an ATP synthase inhibitor, did not affect the amplitude and frequency of rhythmical [Ca2+](i) oscillations. In contrast, two uncoupling agents, carbonylcyanide-3-chlorophenylhydrazone (CCCP) and carbonylcyanide-4-trifluoromethoxyphenylhydrazone (FCCP) effectively abolished rhythmical [Ca2+](i) changes with simultaneous increase in basal [Ca2+](i) in RPV VICs. These data suggest that in RPV VICs mitochondrial Ca2+ handling is important for the generation of rhythmical [Ca2+](i) changes which underlie the spontaneous rhythmical contractile activity in this vessel. (C) 2015 Elsevier Ltd. All rights reserved.