STUDIES ON THE BIOMETRY OF SPERM OF RIVERINE BUFFALO BULLS (BUBALUS BUBALIS)

The present study was conducted to measure various biometric parameters of the Murrah buffalo bulls through computer image analysis. Spermatozoa with intact acrosomes were selected and assessed using an immersion lens (1,000) and standard illumination. The software made it possible to take linear measurements of each spermatozoon: head length, head width, head base, tall length, acrosomal cap length and acrosomal cap width. The results of the morphometric study did not demonstrate the existence of sperm subpopulations. Mean head length, mean head width, mean head base, mean acrosomal cap length and mean acrosomal cap width were 7.59 +/- 0.01 mu m, 4.91 +/- 0.01 mu m, 2.47 +/- 0.01 mu m, 3.46 +/- 0.01 mu m and 4.51 +/- 0.01 mu m, respectively. The mean tail length was 56.14 +/- 0.01 mu m.