Recombinant pea and potato lipoxygenases: Versatile biocatalysts for industry
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作者:
Hughes, RK
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Hughes, RK
Wu, Z
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Wu, Z
Hornostaj, AR
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Hornostaj, AR
Robinson, DS
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Robinson, DS
Hardy, D
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Hardy, D
West, SI
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
West, SI
Owusu-Apenten, RK
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Owusu-Apenten, RK
Fairhurst, SA
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Fairhurst, SA
Chan, M
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Chan, M
Lawson, DM
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Lawson, DM
Casey, R
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机构:John Innes Ctr, Norwich NR4 7UH, Norfolk, England
Casey, R
机构:
[1] John Innes Ctr, Norwich NR4 7UH, Norfolk, England
[2] Univ Leeds, Procter Dept Food Sci, Leeds LS2 9JT, W Yorkshire, England
[3] Biocatalysts Ltd, Pontypridd CF37 5UT, M Glam, Wales
来源:
AGRO FOOD INDUSTRY HI-TECH
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1999年
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10卷
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03期
关键词:
D O I:
暂无
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
We can produce sufficient amounts of specific pea seed and potato tuber lipoxygenase (LOX) by fermentation in Escherichia coli to assess their use in a number of specific applications. This has also allowed us to gain a more fundamental understanding of their differences in behaviour from a comparative analysis of the recombinant enzymes and from the molecular modelling and production and analysis of specific active-site mutants. This article describes some of the properties of the recombinant LOX available and their potential applications in the agrifood, and possibly pharmaceutical industries.
机构:
Inst Super Engn Lisboa, Inst Politecn Lisboa, Area Dept Engn Quim, R Conselheiro Emidio Navarro 1, P-1959007 Lisbon, Portugal
Univ Lisbon, Ctr Quim Estrutural, Inst Super Tecn, Complexo 1,Av Rovisco Pais, P-1049001 Lisbon, PortugalInst Super Engn Lisboa, Inst Politecn Lisboa, Area Dept Engn Quim, R Conselheiro Emidio Navarro 1, P-1959007 Lisbon, Portugal
Sousa, Ana Catarina
Martins, Ligia O.
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机构:
Univ Nova Lisboa, Inst Tecnol Quim & Biol Antonio Xavier, Av Republ, P-2780157 Oeiras, PortugalInst Super Engn Lisboa, Inst Politecn Lisboa, Area Dept Engn Quim, R Conselheiro Emidio Navarro 1, P-1959007 Lisbon, Portugal
Martins, Ligia O.
Robalo, M. Paula
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机构:
Inst Super Engn Lisboa, Inst Politecn Lisboa, Area Dept Engn Quim, R Conselheiro Emidio Navarro 1, P-1959007 Lisbon, Portugal
Univ Lisbon, Ctr Quim Estrutural, Inst Super Tecn, Complexo 1,Av Rovisco Pais, P-1049001 Lisbon, PortugalInst Super Engn Lisboa, Inst Politecn Lisboa, Area Dept Engn Quim, R Conselheiro Emidio Navarro 1, P-1959007 Lisbon, Portugal