Influence of Olive Oil Press Cakes on Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Berk.) Singer (Higher Basidiomycetes) Fruiting Bodies Production and Effect of their Crude Polysaccharides on CCRF-CEM Cell Proliferation

被引:6
|
作者
Gregori, Andrej [1 ]
Kretschmer, Nadine [2 ]
Wagner, Susanne [2 ,3 ]
Boechzelt, Herbert [3 ]
Klinar, Dusan [4 ]
Bauer, Rudolf [2 ]
Pohleven, Franc [5 ]
机构
[1] Zavod Naravoslovje, Ljubljana 1118, Slovenia
[2] Graz Univ, Inst Pharmaceut Sci, Dept Pharmacognosy, A-8010 Graz, Austria
[3] Joanneum Res Forsch Gesell MbH, RESOURCES Inst Water Energy & Sustainabil, A-8010 Graz, Austria
[4] ZRS Bistra Ptuj, Ptuj 2250, Slovenia
[5] Univ Ljubljana, Dept Wood Sci & Technol, Biotech Fac, Ljubljana 1000, Slovenia
关键词
medicinal mushrooms; Lentinus edodes; Shiitake mushroom; polysaccharides; olive oil press cakes; cytotoxicity; CCRF-CEM leukemia cells; BIOLOGICAL EFFICIENCY; CULTIVATION; SUBSTRATE; CULTURE; GROWTH;
D O I
10.1615/IntJMedMushr.v14.i4.100
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lentinus edodes (Berk.) Singer fruiting bodies were cultivated on substrates composed of beech sawdust, wheat bran, and calcium sulfate hemihydrate (gypsum), containing different proportions of olive oil press cakes (OOPC). We determined the influence of OOPC on fruiting bodies production and proliferation of CCRF-CEM leukemia cells. A negative influence of OOPC on mycelia growth and maturation was noticed. When growth medium contained 80% OOPC, fruiting bodies ceased forming. To investigate the cytotoxicity on CCRF-CEM cells in vitro, cells were treated with crude polysaccharides extracted from L. edodes fruiting bodies. Also in this case a negative correlation between OOPC content and cytotoxicity was found.
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页码:419 / 424
页数:6
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