Data on Western blot and ELISA analysis of medaka (Oryzias latipes) follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) using recombinant proteins expressed with Pichia pastoris

The gonadotropins follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) play essential roles in vertebrate reproduction. This article presents data on molecular weight validation of recombinant medaka (Oryzias latipes) (md) gonadotropins Fsh beta (mdFsh beta), Lh beta (mdLh beta), Fsh beta alpha (mdFsh beta alpha), and Lh beta alpha (mdLh beta alpha) generated by Pichia pastoris, as well as data on a validation of produced antibodies against Fsh beta and Lh beta by Western blot analysis. Furthermore, the article includes data on Fsh and Lh protein levels in male medaka pituitaries using recombinant mdFshpoi and mdLh beta alpha within enzyme-linked immunosorbent assays (ELISA5), in which protein amounts were analyzed related to body weight and age of the fish. This dataset is associated with the research article entitled "Medaka Follicle-stimulating hormone (Fsh) and Luteinizing hormone (Lh): Developmental profiles of pituitary protein and gene expression" (Burow et al., in press). (C) 2019 Published by Elsevier Inc.