Polymerase chain reaction engineering

被引：0
|
作者：
Hsu, JT
Das, S
Mohapatra, S
机构：
[1] Biopharmaceutical Technol. Institute, Department of Chemical Engineering, Lehigh University, Bethlehem
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1997年 / 55卷 / 02期
关键词：
polymerase chain reaction; DNA; mathematical simulation;
D O I：
10.1002/(SICI)1097-0290(19970720)55:2<359::AID-BIT13>3.0.CO;2-C
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A mathematical model for polymerase chain reaction (PCR) is developed, taking into account the three steps in this process: melting of DNA; primer annealing; and DNA synthesis (polymerization). Activity and deactivation of the polymerase enzyme as a function of temperature is incorporated in the kinetic model to get a better understanding of the amplification of DNA. Computer simulation of the model is carried out to determine the effects of various parameters, such as the cycle number, initial DNA concentration (copynumber), initial enzyme concentration, extension time, temperature ramp, and enzyme deactivation on the DNA generation. (C) 1997 John Wiley & Sons, Inc.
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页码：359 / 366
页数：8
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