Discrimination between Native and Tn6010-Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy

被引：19

作者：

Guillard, Thomas ^{[1
,2
]}

Lebreil, Anne-Laure ^{[2
]}

Hansen, Lars Hestbjerg ^{[3
]}

Kisserli, Aymric ^{[4
,5
]}

Berger, Sibel ^{[6
]}

Lozniewski, Alain ^{[6
,7
]}

Alauzet, Corentine ^{[6
,7
]}

de Champs, Christophe ^{[1
,2
]}

机构：

[1] CHU Reims, Hop Robert Debre, Lab Bacteriol Virol Hyg, Reims, France

[2] Univ Reims, UFR Med, EA ERA 4687, SFR CAP Sante, Reims, France

[3] CHU Reims, Hop Robert Debre, Immunol Lab, Reims, France

[4] Univ Reims, UFR Med, EA 4682, SFR CAP Sante, Reims, France

[5] Aarhus Univ, Dept Environm Sci, Roskilde, Denmark

[6] CHU Nancy, Lab Bacteriol, Nancy, France

[7] Univ Lorraine, EA Stress Immunite Pathogenes 7300, Vandoeuvre Les Nancy, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2015年 / 59卷 / 09期

关键词：

MULTIDRUG EFFLUX PUMP; ESCHERICHIA-COLI; QUINOLONE RESISTANCE; EXTENDED-SPECTRUM; PLASMID; PREVALENCE; PNEUMONIAE; OLAQUINDOX;

D O I：

10.1128/AAC.00669-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB.

引用

页码：5838 / 5840

页数：3

共 5 条

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