3D Printing-Assisted Rapid Prototyping and Optimization: Development of a Novel Small Intestinal Cannula for Equine Research

In the field of nutrition research, the surgical placement of intestinal cannulas in animal models is a commonly used method for assessing nutrient absorption along the digestive tract. This technique allows researchers to obtain intestinal fluid at key checkpoints along the digestive tract. Cannulation studies have been limited by the availability of suitable cannulas for different animal models. This study discusses the creation of a novel cannula that was fabricated using 3D-printed molds and moldable silicone putty. CAD software was used to design the cannula, and 3D-printed models allowed our clinical researchers to assess physical models for suitability. A negative of the final model was created by CAD, sectioned into three pieces to serve as a mold, and then 3D printed. This mold was filled with silicone putty, compressed using vices, and allowed to set. The resulting cannula was removed from the mold and trimmed of excess material to create the final product. These cannulas were then surgically implanted as part of ongoing equine nutrition research. This technique allowed for the rapid development and optimization of custom cannulas, without the prohibitively high costs, expertise requirements, and timeline associated with traditional rubber molding techniques.