Single nucleotide polymorphism markers for genotyping hawksbill turtles (Eretmochelys imbricata)

被引:5
|
作者
Banerjee, Shreya M. [1 ]
Komoroske, Lisa M. [1 ,2 ]
Frey, Amy [2 ]
Hancock-Hanser, Brittany [2 ]
Morin, Phillip A. [2 ]
Archer, Frederick I. [2 ]
Roden, Suzanne [2 ]
Gaos, Alexander [2 ,3 ]
Liles, Michael J. [4 ]
Dutton, Peter H. [2 ]
机构
[1] Univ Massachusetts, Dept Environm Conservat, 160 Holdsworth Way, Amherst, MA 01003 USA
[2] NOAA, Southwest Fisheries Sci Ctr, Natl Marine Fisheries Serv, 8901 La Jolla Shores Dr, La Jolla, CA 92037 USA
[3] NOAA, Joint Inst Marine & Atmospher Res, Pacific Isl Fisheries Sci Ctr, Natl Marine Fisheries Serv,Marine Turtle Biol & A, Honolulu, HI 96818 USA
[4] Asociac ProCosta, Poligono F,33, San Salvador, El Salvador
关键词
DNA capture array; Sea turtle; Conservation; SNP; Genotyping by sequencing; POPULATION-STRUCTURE; PHYLOGEOGRAPHY; MITOCHONDRIAL; CONSERVATION; MTDNA;
D O I
10.1007/s12686-019-01112-z
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Assessing population connectivity and identifying conservation units are critical to formulating effective management plans for marine turtles. We combined a multiplexed targeted enrichment capture technique and high-throughput sequencing to discover single nucleotide polymorphisms (SNPs) and to genotype hawksbill turtles (Eretmochelys imbricata) to examine population structure. Our approach discovered 455 potential informative SNPs for hawksbill turtles. We optimized 25 of the 455 SNPs discovered and used them to identify preliminary evidence of hawksbill population structure, suggesting that these markers will be informative in discerning conservation units when applied to larger sample sizes in future studies.
引用
收藏
页码:353 / 356
页数:4
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