Comparison of in vitro viability methods for Cryptosporidium oocysts

被引：12
|
作者：
Vande Burgt, Nathan H. [1 ]
Auer, Agathe [1 ]
Zintl, Annetta [1 ]
机构：
[1] Univ Coll Dublin, Sch Vet Med, Dublin 4, Ireland
来源：
EXPERIMENTAL PARASITOLOGY | 2018年 / 187卷
关键词：
PMA-qPCR; Polymer adhesion; Fluorescence in-situ hybridization; Excystation; Propidium iodide; PARVUM OOCYSTS; PROPIDIUM MONOAZIDE; RIBOSOMAL-RNA; GIARDIA-LAMBLIA; PCR; EXCYSTATION; WATER; INFECTIVITY; TIME; DISINFECTION;
D O I：
10.1016/j.exppara.2018.03.002
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of infection. In this study, we compared the ability of various in vitro methods to discriminate viable from non-viable oocysts, including excystation, DAPI/PI staining, RNA FISH, PMA-qPCR and a novel polymer slide adhesion method. With the notable exception of our in vitro excystation protocol, all methods were found to be useful for identifying viable oocysts. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：30 / 36
页数：7
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