Bioconversion of phenylpyruvic acid to L-phenylalanine by mixed-gel immobilization of Escherichia coli EP8-10

被引:7
|
作者
Hu, Yonghong [1 ]
Tang, Tianyu [1 ]
Yang, Wenge [1 ]
Zhou, Hua [1 ]
机构
[1] Nanjing Univ Technol, Coll Life Sci & Pharm, Nanjing 210009, Peoples R China
关键词
Mixed-gel immobilization; Transaminase; Bioconversion; kappa-Carrageenan; L-Phenylalanine; Gelatin; L-ASPARTIC ACID; COUPLING REACTIONS;
D O I
10.1016/j.procbio.2008.09.020
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A mixed-gel of K-carrageenan and gelatin was used in L-phenylalanine production. The mixed-gel, containing 87.5% K-carrageenan and 12.5% gelatin [the total gel concentration was 4 wt%], showed the best performance and was selected for further study with Escherichia coli EP8-10. The optimum pH and temperature were 8.5 and 37 degrees C, respectively. The effects of trehalose and Mg2+ were studied in the mixed-gel immobilization. Their optimum concentrations were 5 x 10(-2) and 2 x 10(-3) mol/L, respectively. Under the optimal conditions, 98.3% of the phenylpyruvic acid (PPA) was converted to L-phenylalanine. The activity recovery of the transaminase enzyme in the mixed-gel immobilization was higher than that in single K-carrageenan immobilization, which was 93.6%. The total PPA conversion rate was over 80% in all 15 batches, suggesting great sustainability in the mixed-gel immobilization. The maximum reaction rate (r(max)) was calculated to be 4.75 x 10(-2) mol/(Lg h). Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:142 / 145
页数:4
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