Specific tools for targeting and expression in Muller glial cells

被引：40

作者：

Pellissier, Lucie P. ^{[1
]}

Hoek, Robert M. ^{[1
]}

Vos, Rogier M. ^{[1
]}

Aartsen, Wendy M. ^{[1
]}

Klimczak, Ryan R. ^{[2
,3
]}

Hoyng, Stefan A. ^{[4
]}

Flannery, John G. ^{[2
,3
]}

Wijnholds, Jan ^{[1
]}

机构：

[1] Netherlands Inst Neurosci, Dept Neuromed Genet, Amsterdam, Netherlands

[2] Univ Calif Berkeley, Dept Mol & Cellular Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Helen Wills Neurosci Inst, Berkeley, CA 94720 USA

[4] Netherlands Inst Neurosci, Dept Neuroregenerat, Amsterdam, Netherlands

来源：

MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT | 2014年 / 1卷

关键词：

RETINALDEHYDE-BINDING PROTEIN; CD44; GENE-EXPRESSION; IMMEDIATE-EARLY GENE; ADENOASSOCIATED VIRUS; TRANSDUCTION; PROMOTER; INTERLEUKIN-1-BETA; LOCALIZATION; RETINA; VECTOR;

D O I：

10.1038/mtm.2014.9

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Despite their physiological roles, Muller glial cells are involved directly or indirectly in retinal disease pathogenesis and are an interesting target for therapeutic approaches for retinal diseases and regeneration such as CRB1 inherited retinal dystrophies. In this study, we characterized the efficiency of adeno-associated virus (AAV) capsid variants and different promoters to drive protein expression in Muller glial ces. ShH10Y and AAV9 were the most powerful capsids to infect mouse Muller glial cells. Retinaldehydebinding protein 1 (RLBP1) promoter was the most powerful promoter to transduce Muller glial cells. ShH10Y capsids and RLBP1 promoter targeted human Muller glial cells in vit. We also developed and tested smaller promoters to express the large CRB1 gene via AAV vectors. Minimal cytomegalovirus (CMV) promoter allowed expression of full-length CRB1 protein in Muller glial cells. In summary, ShH10Y and AAV9 capsids, and RLBP1 or minimal CMV promoters are of interest as specific tools to target and express in mouse or human Muller glial cells.

引用

页数：9

共 50 条

[1] Retroviral targeting of Muller glial cells in the developing chick retina
Lewis, R
Buzzi, WR
Linser, PJ
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1996, 37 (03) : 2973 - 2973
[2] Muller glial cells modulate the expression of vitreous matrix components
Akamine, Priscilla Sayami
Del Debbio, Carolina B.
Teles Rodrigues, Raquel Cecilia
Fuziwara, Cesar Seigi
Kimura, Edna Teruko
Hamassaki, Dania E.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2015, 56 (07)
[3] The expression of Toll-like receptors in murine Muller cells, the glial cells in retina
Lin, Xiaomin
Fang, Dan
Zhou, Hongyan
Su, Shao Bo
NEUROLOGICAL SCIENCES, 2013, 34 (08) : 1339 - 1346
[4] Muller glial cells in anuran retina
Bringmann, A
Skatchkov, SN
Pannicke, T
Biedermann, B
Wolburg, H
Orkand, RK
Reichenbach, A
MICROSCOPY RESEARCH AND TECHNIQUE, 2000, 50 (05) : 384 - 393
[5] Muller Glial Cells in Retinal Disease
Bringmann, Andreas
Wiedemann, Peter
OPHTHALMOLOGICA, 2012, 227 (01) : 1 - 19
[6] EXPRESSION OF FUNCTIONAL NGF RECEPTORS BY HUMAN MULLER GLIAL-CELLS IN CULTURE
IKEDA, T
PURO, DG
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1994, 35 (04) : 1496 - 1496
[7] Porcine Muller glial cells increase expression of BKCa channels in retinal detachment
Bringmann, Andreas
Iandiev, Ianors
Pannicke, Thomas
Wurm, Antje
Buehner, Eva
Reichenbach, Andreas
Wiedemann, Peter
Uhlmann, Susann
CURRENT EYE RESEARCH, 2007, 32 (02) : 143 - 151
[8] Expression of glial fibrillary acidic protein in primary cultures of human Muller cells
Lupien, C
Brenner, M
Guérin, SL
Salesse, C
EXPERIMENTAL EYE RESEARCH, 2004, 79 (03) : 423 - 429
[9] Expression of potassium channels during postnatal differentiation of rabbit Muller glial cells
Bringmann, A
Biedermann, B
Reichenbach, A
EUROPEAN JOURNAL OF NEUROSCIENCE, 1999, 11 (08) : 2883 - 2896
[10] Gene Expression Changes in Retinal Muller (Glial) Cells Exposed to Elevated Pressure
Xue, Wei
Du, Pan
Lin, Simon
Dudley, V. Joseph
Hernandez, M. Rosario
Sarthy, Vijay P.
CURRENT EYE RESEARCH, 2011, 36 (08) : 754 - 767

← 1 2 3 4 5 →