Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli

被引：10
|
作者：
Graupner, S [1 ]
Wackernagel, W [1 ]
机构：
[1] UNIV OLDENBURG,FACHBEREICH BIOL,D-26111 OLDENBURG,GERMANY
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 05期
关键词：
D O I：
10.1128/AEM.62.5.1839-1841.1996
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than that of the expression plasmid. Evidence that plasmid-like structures are formed in H. polymorpha and that their formation is stimulated by DNA damage is presented in this study.
引用
收藏
页码：1839 / 1841
页数：3
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