Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector

被引：17

作者：

Qi, Jing ^{[1
]}

Shao, Yonghong ^{[1
]}

Liu, Lixin ^{[2
]}

Wang, Kaige ^{[1
]}

Chen, Tongsheng ^{[3
]}

Qu, Junle ^{[1
]}

Niu, Hanben ^{[1
]}

机构：

[1] Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China

[2] Xidian Univ, Sch Tech Phys, Xian 710071, Peoples R China

[3] S China Normal Univ, Inst Laser Life Sci, Guangzhou 510631, Peoples R China

来源：

OPTICS LETTERS | 2013年 / 38卷 / 10期

基金：

中国国家自然科学基金;

关键词：

2-PHOTON MICROSCOPY; ULTRAFAST; CELLS;

D O I：

10.1364/OL.38.001697

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a fast and flexible fluorescence lifetime imaging microscopy which uses a two-dimensional acousto-optic deflector to achieve fast beam scanning across the sample and provides random access to the regions of interests (ROI). Experimental results using standard fluorescent dye and biological samples show that this system can make addressable fluorescence lifetime measurements and perform fast and flexible fluorescence lifetime imaging particularly to the discontinuous ROI in the sample. (C) 2013 Optical Society of America

引用

页码：1697 / 1699

页数：3

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