The Escherichia coli NadR regulator is endowed with nicotinamide mononucleotide adenylyltransferase activity

被引：68
|
作者：
Raffaelli, N
Lorenzi, T
Mariani, PL
Emanuelli, M
Amici, A
Ruggieri, S
Magni, G
机构：
[1] Univ Ancona, Fac Med, Ist Biochim, I-60131 Ancona, Italy
[2] Univ Ancona, Dipartimento Biotecnol Agr & Ambientali, I-60131 Ancona, Italy
来源：
JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 17期
关键词：
D O I：
10.1128/JB.181.17.5509-5511.1999
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The first identification and characterization of a catalytic activity associated with NadR protein is reported. A computer-aided search for sequence similarity revealed the presence in NadR of a 29-residue region highly conserved among known nicotinamide mononucleotide adenylyltransferases. The Escherichia coli nadR gene was cloned into a T7-based vector and overexpressed. In addition to functionally specific DNA binding properties, the homogeneous recombinant protein catalyzes NAD synthesis from nicotinamide mononucleotide and ATP.
引用
收藏
页码：5509 / 5511
页数：3
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