QDs-labeled microspheres for the adsorption of rabbit immunoglobulin G and fluoroimmunoassay

被引：42
|
作者：
Ma, Qiang [1 ]
Song, Tian-You [2 ]
Yuan, Pingfan [1 ]
Wang, Chao [1 ]
Su, Xing-Guang [1 ]
机构：
[1] Jilin Univ, Dept Analyt Chem, Coll Chem, Changchun 130012, Peoples R China
[2] Jilin Univ, Dept Inorgan Chem, Coll Chem, Changchun 130012, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2008年 / 64卷 / 02期
基金：
中国国家自然科学基金;
关键词：
QDs; fluorescent microspheres; adsorption; fluoroimmunoassay;
D O I：
10.1016/j.colsurfb.2008.01.027
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
This paper presents a study on the adsorption of rabbit immunoglobulin G onto CdTe quantum dots (QDs)/polystyrene microspheres. The adsorption appears to be sensitive to pH conditions and ionic strength. Maximum adsorption for protein was obtained near the isoelectric point. Adsorption isotherm analysis demonstrated that the electrostatic interaction plays an important role in the adsorption of protein. The thickness of adsorbed layer calculated from the maximal adsorption amounts (q(m)) is 6.5 nm, which indicates that the rabbit IgG molecules exist between the side-on and end-on mode in the monolayer. The bio-functional rabbit IgG/fluorescent microspheres were further used for the detection of antibody in fluoroimmunoassays. This approach allowed detection of goat anti-rabbit IgG in the range of 1-100 ng/mL. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：248 / 254
页数：7
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