Effect of Oxymatrine on H2O2 Resistance in L6 Rat Myoblast Cells

Effect of oxymatrine on the H2O2 of L-6 rat myoblast cells was investigated in this study. The hydrogen peroxide(H2O2) was used to establish the H2O2-induced apoptosis model by L-6 rat myoblast cells that were treated with 0.3, 0.15, 0.75 g/L oxymatrine. The survival rate was measured by MTT method, cell cycles and apoptosis rate of L6 rat myoblast cells were detected by flow cytometry, the injury degree was determined by apoptosis cytochemistry fluorescent antibody of Bcl-2 and Bax, DAPI staining and HE staining. The protein differences were examined by Western blot. Results showed that survival rate of L-6 myoblast cells was degraded and the apoptosis rate was increased by damaging H2O2. The survival rate of L-6 myoblast cells was heightened by oxymatrine of each dose. They could induce the increase in expression of Bcl-2 and the decrease in expression of Bax. Its degree of protection was increased with raising the dosage of oxymatrine and the protective effect of 0.3 g/L dosage is obvious. The protective effect of 0.15, 0.75 g/L oxymatrine were secondary. The main physiological and biochemical effects of oxymatrine was related to protect rat myoblast apoptosis model through NF kappa B signal transduction. These results implied that oxymatrine may have the potential as a new antioxidant treatment medicine.