Performance analysis of microchip electrophoresis with portable UV absorbance detection system

This paper presents a portable miniaturized integration UV-VIS absorbance detection system for the microchip electrophoresis (MCE). The TOCON_nano high sensitive SiC pre-amplified UV Sensor (Sglux GmbH, Ostendstrasse, Berlin, Germany) is used to measure extremely low UV radiation intensities in the nanowatt scale. This detection system volume is 280 x200x150 mm(3), the weight is less than 2Kg. The PMMA was used as the MCE substrate materials. And the electrophoresis microchannels are fabricated with the CNC milling, have the cross section area of 150 mu m x150 mu m. With this detection system, the detection limit for the Cl- electrophoresis can reach 10(-10)mol at the indirect UV-VIS detection mode. Two inorganic ions Cl- and SO42- could reach the baseline separation in 3 min.