Inducible control of gene expression with destabilized Cre

被引：2

作者：

Sando, Richard, III ^{[1
,2
]}

Baumgaertel, Karsten ^{[1
]}

Pieraut, Simon ^{[1
]}

Torabi-Rander, Nina ^{[1
]}

Wandless, Thomas J. ^{[3
]}

Mayford, Mark ^{[1
]}

Maximov, Anton ^{[1
]}

机构：

[1] Scripps Res Inst, Dorris Neurosci Ctr, Dept Mol & Cellular Neurosci, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Kellogg Sch Sci & Technol, La Jolla, CA 92037 USA

[3] Stanford Univ, Dept Chem & Syst Biol, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2013年 / 10卷 / 11期

基金：

美国国家卫生研究院;

关键词：

SYNAPTIC-TRANSMISSION; MICE; NEURONS; MEMORY; INHIBITION; PLASTICITY; BRAIN;

D O I：

10.1038/NMETH.2640

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A cute manipulation of gene and protein function in the brain is essential for understanding the mechanisms of nervous system development, plasticity and information processing. Here we describe a technique based on a destabilized Cre recombinase (DDDD-Cre) whose activity is controlled by the antibiotic trimethoprim (TMTMP). We show that DDDD-Cre triggers rapid TMTMP-dependent recombination of loxP-flanked ('floxed') alleles in mouse neurons in vivo and validate the use of this system for neurobehavioral research.

引用

页码：1085 / 1088

页数：4

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