NT-4/5 protects against adrenalectomy-induced apoptosis of rat hippocampal granule cells

被引：15
|
作者：
Qiao, XX [1 ]
Hughes, PE [1 ]
Venero, JL [1 ]
DugichDjordjevic, MM [1 ]
Nichols, NR [1 ]
Hefti, F [1 ]
Knusel, B [1 ]
机构：
[1] UNIV SO CALIF,ANDRUS GERONTOL CTR,LOS ANGELES,CA 90098
来源：
NEUROREPORT | 1996年 / 7卷 / 02期
关键词：
neurotrophic factors; NT-4/5; NT-3; apoptosis; neuroprotection; Alzheimer's disease; Huntington's disease; dentate gyrus; neurodegeneration; corticosterone hormones;
D O I：
10.1097/00001756-199601310-00071
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
ADRENALECTOMY (ADX) in rats has been shown to induce apoptosis of hippocampal granule cells. We tested whether neurotrophins are able to protect hippocampal neurons in this neurodegeneration model. Acid fuchsin stain was used to identify pyknotic cells in ADX rats treated for 4 days with NT-3, NT-4-5 or cytochrome-C, as a control protein. Cytochrome-C injections slightly decreased cell death on the ipsilateral side. NT-3 did not further promote this effect. Significantly less cell death was observed bilaterally in hippocampus treated with NT-4/5. TUNEL end labeling also confirmed the results. Our results demonstrated that NT-4/5, but not NT-3, promotes hippocampal neuron survival in adrenalectomized rats. They further show that injections of a control solution can induce a local protective effect.
引用
收藏
页码：682 / 686
页数：5
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