Fuchsin fluorescence in Mycobacterium tuberculosis:: The Ziehl-Neelsen stain in a new light

被引:9
|
作者
Shapiro, Howard M. [1 ,2 ]
Haenscheid, Thomas [3 ]
机构
[1] Ctr Microbial Cytometry, W Newton, MA 02465 USA
[2] Howard M Shapiro MD PC, W Newton, MA 02465 USA
[3] Hosp Santa Maria, Fac Med, Inst Microbiol, P-1649028 Lisbon, Portugal
关键词
fluorescence microscopy; fuchsin; tuberculosis diagnosis; Ziehl-Neelsen stain;
D O I
10.1016/j.mimet.2008.04.005
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Tuberculosis (TB) is often diagnosed by observation of reddish pink fuchsin-stained Mycobacterium tuberculosis (MTB) in Ziehl-Neelsen (Z-N) stained smears by transmitted light microscopy. MTB too faintly stained with fuchsin to be seen by transmitted light may be detected by their green-excited orange-red fluorescence; this finding may be clinically relevant. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:119 / 120
页数:2
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