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Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures
被引:111
|作者:
Mannina, Giorgio
[1
]
Presti, Dario
[1
]
Montiel-Jarillo, Gabriela
[2
]
Eugenia Suarez-Ojeda, Maria
[2
]
机构:
[1] Univ Palermo, Dipartimento Ingn, Viale Sci,Ed 8, Palermo, Italy
[2] Univ Autonoma Barcelona, Dept Chem Biol & Environm Engn, GENOCOV Res Grp, Sch Engn,Escola Engn, Edifici Q Campus UAB, E-08193 Barcelona, Spain
关键词:
Bioplastic;
Water resource recovery;
PHA;
Wastewater treatment;
POLY(3-HYDROXYBUTYRATE) PRODUCTION;
MICROSTRUCTURE;
PERFORMANCE;
ENRICHMENT;
EFFICIENT;
BIOMASS;
D O I:
10.1016/j.biortech.2019.03.037
中图分类号:
S2 [农业工程];
学科分类号:
0828 ;
摘要:
A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 +/- 8% and 100 +/- 5%, respectively) was obtained when using NH4-Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 degrees C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.
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页码:361 / 369
页数:9
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