Interactions between bovine serum albumin and alginate: An evaluation of alginate as protein carrier

被引:161
|
作者
Zhao, Yinyan [1 ]
Li, Fuyue [1 ]
Carvajal, M. Teresa [2 ]
Harris, Michael T. [1 ]
机构
[1] Purdue Univ, Sch Chem Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Dept Ind & Phys Pharm, W Lafayette, IN 47907 USA
基金
美国国家科学基金会;
关键词
Intermolecular interactions; Complexes; Denaturation; Carboxyl groups; Dissociation; LIGHT-SCATTERING; POLYSACCHARIDE INTERACTIONS; BETA-LACTOGLOBULIN; WHEY PROTEINS; SYSTEMS; RAMAN; SPECTROSCOPY; DENATURATION; SURFACTANTS; COMPLEXES;
D O I
10.1016/j.jcis.2008.12.048
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The intermolecular interactions between the model protein, bovine serum albumin (BSA) and a biocompatible polysaccharicle, sodium alginate, have been investigated. Both the native BSA and the heat pre-denatured BSA were utilized to study, in parallel, the effect of protein conformational change during the protein-alginate complex formation. In this work, a comparison was performed between the native BSA and the heat-denatured BSA incubated sodium alginate mixtures by using zeta potential analyzer, dynamic light scattering (DLS) and turbidimetric analysis of the systems in combination with protein conformational tools, Fourier transform infrared spectroscopy (FT-IR) and size exclusion chromatography (SE-HPLC). The experimental results demonstrate that the intermolecular chain associations were formed between alginate chains and protein molecules in either the native form or the heat pre-denatured form, mainly driven by the electrostatic interactions between the oppositely charged amino acids and the anionic polysaccharide macromolecules. However, the majority of BSA was recovered from the dissociation of protein-alginate complexes and maintained its secondary structure and conformational property. Therefore, alginate is promising as a bioactive compound carrier. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:345 / 353
页数:9
相关论文
共 50 条
  • [1] Interactions between Bovine Serum Albumin and Oxidized Sodium Alginate in Solution
    Gao, Chunmei
    Liu, Mingzhu
    Chen, Jun
    Chen, Chen
    JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2011, 22 (12) : 1639 - 1650
  • [2] Interactions in bovine serum albumin-calcium alginate gel systems
    Neiser, S
    Draget, KI
    Smidsrod, O
    FOOD HYDROCOLLOIDS, 1999, 13 (06) : 445 - 458
  • [3] Structural, thermal and rheological characterization of bovine serum albumin binding with sodium alginate
    Xu, Xi
    Han, Qianqian
    Shi, Juanjuan
    Zhang, Hongmei
    Wang, Yanqing
    JOURNAL OF MOLECULAR LIQUIDS, 2020, 299 (299)
  • [4] In vitro release of bovine serum albumin from alginate/HPMC hydrogel beads
    Nochos, Argyrios
    Douroumis, Dionysios
    Bouropoulos, Nikolaos
    CARBOHYDRATE POLYMERS, 2008, 74 (03) : 451 - 457
  • [5] Sodium Alginate Microneedle Arrays Mediate the Transdermal Delivery of Bovine Serum Albumin
    Demir, Yusuf K.
    Akan, Zafer
    Kerimoglu, Oya
    PLOS ONE, 2013, 8 (05):
  • [6] Influence of DNA, alginate, lysozyme and bovine serum albumin on sodium silicate condensation
    Coradin, T
    Coupé, A
    Livage, J
    BIOLOGICAL BIOMIMETIC MATERIALS-PROPERTIES TO FUNCTION, 2002, 724 : 147 - 152
  • [7] Interactions of hydrophobically modified hyaluronan carrier with bovine serum albumin
    Ondreas, Frantisek
    Sita, Jaroslav
    Cepa, Martin
    Svecova, Eliska
    Velebny, Vladimir
    APPLIED SURFACE SCIENCE, 2022, 593
  • [8] Alginate as an antiglycating agent for human serum albumin
    Sattarahmady, Naghmeh
    Khodagholi, Fariba
    Moosavi-Movahedi, Ali A.
    Heli, Hossein
    Hakimelahi, Gholam H.
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2007, 41 (02) : 180 - 184
  • [9] Preparation and release behavior of carboxymethylated chitosan/alginate microspheres encapsulating bovine serum albumin
    Zhang, L
    Guo, J
    Peng, XH
    Jin, Y
    JOURNAL OF APPLIED POLYMER SCIENCE, 2004, 92 (02) : 878 - 882
  • [10] SAME OBSERVATIONS ON THE NATURE OF HEATED MIXTURES OF BOVINE SERUM-ALBUMIN WITH AN ALGINATE AND A PECTIN
    KELLY, R
    GUDO, ES
    MITCHELL, JR
    HARDING, SE
    CARBOHYDRATE POLYMERS, 1994, 23 (02) : 115 - 120