Microscale thioacidolysis method for the rapid analysis of β-O-4 substructures in lignin

被引：52
|
作者：
Yamamura, Masaomi [1 ]
Hattori, Takefumi [2 ]
Suzuki, Shiro [1 ]
Shibata, Daisuke [3 ]
Umezawa, Toshiaki [1 ,4 ]
机构：
[1] Kyoto Univ, Res Inst Sustainable Humanosphere, Kyoto 6110011, Japan
[2] Univ Tokushima, Inst Socioarts & Sci, Tokushima 7708502, Japan
[3] Kazusa DNA Res Inst, Kisarazu, Chiba 2920818, Japan
[4] Kyoto Univ, Inst Sustainabil Sci, Kyoto 6110011, Japan
来源：
PLANT BIOTECHNOLOGY | 2012年 / 29卷 / 04期
关键词：
High-throughput; lignin; microscale; thioacidolysis; beta-O-4; substructure; HIGH-THROUGHPUT DETERMINATION; DFRC METHOD; BIOSYNTHESIS; CLEAVAGE; DEGRADATION;
D O I：
10.5511/plantbiotechnology.12.0627a
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Thioacidolysis is a method to detect the beta-O-4 substructures of lignin, and has been employed as a diagnostic test for the presence of lignin. However, the conventional thioacidolysis protocol is low-throughput and is a bottleneck in the characterization of lignins in a large number of samples such as transgenic lines. Recently, a rapid analysis protocol for thioacidolysis was reported. In this study, we modified mainly the work-up process. Our microscale protocol showed higher yields of thioacidolysis products than the conventional protocol.
引用
收藏
页码：419 / 423
页数：5
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