Genotyping of Asparagus (Asparagus officinalis L.) Cultivars with SSR Markers

被引：2
|
作者：
Takeuchi, Y. [1 ]
Sakaguchi, Y. [1 ]
Ozaki, Y. [2 ]
Okubo, H. [3 ]
机构：
[1] Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Hort Sci Lab, Fukuoka 812, Japan
[2] Kyushu Univ, Fac Agr, Lab Agr Ecol, Fukuoka 812, Japan
[3] Kyushu Univ, Fac Agr, Hort Sci Lab, Fukuoka 812, Japan
来源：
XII INTERNATIONAL ASPARAGUS SYMPOSIUM | 2012年 / 950卷
关键词：
expressed sequence tag (EST); molecular markers; Mendelian inheritance; linkage arrangement; genotype; SEX; IDENTIFICATION; PROGENIES; CROSSES;
D O I：
10.17660/ActaHortic.2012.950.17
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Inheritance of SSR markers, linkage arrangement of the SSR loci and genotyping of asparagus cultivars of different ploidies were investigated. The segregation patterns of SSR markers in the progenies of 'Hokkai 100' x 'UC157F(1)', 'Welcome' x 'Welcome' and 'Gold Schatz' x 'Hokkai 100' fitted with Mendelian inheritance in six EST-SSR loci, AG2, AG3, AG7, AG10, TC1 and TC7. One linkage combination of 'Asp1-T7sp marker (Nakayama et al., 2006), AG3' was recognized with the recombination fractions of 0.138 at 1% level. There were 34 alleles in the six loci ranging from four to eight alleles per locus. SSR genotypes of diploid, triploid and tetraploid cultivars were distinguished, and parental genotypes of the diploid clonal hybrid cultivars were estimated from their segregation patterns.
引用
收藏
页码：165 / 172
页数：8
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