DETERMINATION OF 2,4-DICHLOROPHENOXYACETIC ACID AND ITS METABOLITES BY IMMUNOAFFINITY CHROMATOGRAPHY IN PLANT MATRICES

Monoclonal antibodies against widely used herbicide 2,4-D were produced by hybridomas from fusion of murine myeloma cells and spleencells isolated from BALB/c mice immunized with hapten conjugated via the carboxyl group to thyroglobulin. The monoclonal antibodies was subsequently characterized by competitive ELISA employing rabbit anti-mouse immunoglobulin to capture antibody from the ascites fluid and the peroxidase conjugate as a detection component. The cross-reactivity of the antibodies was defined on the bases of competitive study with other 2,4-D structural analogs. The selected broad-specific monoclonal antibodies (E2/G2) were coupled to Affi-Gel (R) 10 and immunoaffinity columns were prepared. These columns were subsequently used for immunoaffinity isolation of 2,4-D and its metabolites from plant extracts.