The effect of increased centrifugation temperature on the quality of red-blood-cell concentrates of automated whole blood processing

Background and Objectives There are manual and automated methods to separate whole blood (WB) available. The Atreus whole blood processing system is an automated method, which combines centrifugation and expression of components into a single device. A major difference to conventional methods is that centrifugation temperature is not controlled at 22 degrees C. The aim of this study was to examine the influence of increased centrifugation temperatures on the quality of red-blood-cell concentrates (RCC) after active cooling of WB prior to processing. Materials and Methods A total of 28 WB were processed: 16 at centrifugation temperatures of up to 28 degrees C (1st protocol) and 12 at 34 degrees C (2nd protocol). RCC quality parameters were tested weekly for 42days. Results Red-blood-cell concentrates (RCC) quality complied with the European and German guidelines. Haemolysis was not significantly different throughout storage. Significant statistical differences were detected between both protocols in potassium concentration at the end of storage and in ATP levels at the day of processing. Conclusion Centrifugation temperatures of up to 34 degrees C are well tolerated by the red blood cells with minimal interference with the RCC quality parameters.