A conserved motif present in a class of helix-loop-helix proteins activates transcription by direct recruitment of the SAGA complex

被引:109
|
作者
Massari, ME
Grant, PA
Pray-Grant, MG
Berger, SL
Workman, JL
Murre, C [1 ]
机构
[1] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA
[2] Penn State Univ, Dept Biochem & Mol Biol, Howard Hughes Med Inst, University Pk, PA 16802 USA
[3] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA
关键词
D O I
10.1016/S1097-2765(00)80188-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The class I helix-loop-helix (HLH) proteins, which include E2A, HEB, and E2-2, have been shown to be required for lineage-specific gene expression during T and B lymphocyte development. Additionally, the E2A proteins function to regulate V(D)J recombination, possibly by allowing access of variable region segments to the recombination machinery. The mechanisms by which E2A regulates transcription and recombination, however, are largely unknown. Here, we identify a novel motif, LDFS, present in the vertebrate class I HLH proteins as well as in a yeast HLH protein that is essential for transactivation. We provide both genetic and biochemical evidence that the highly conserved LDFS motif stimulates transcription by direct recruitment of the SAGA histone acetyltransferase complex.
引用
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页码:63 / 73
页数:11
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