A comparison of recovery methods of rhamnolipids produced by Pseudomonas aeruginosa

被引：32
|
作者：
Shah, Mansoor Ul Hassan [1 ]
Sivapragasam, Magaret [2 ]
Moniruzzaman, Muhammad [1 ,2 ]
Yusup, Suzana Bt [1 ]
机构：
[1] Univ Teknol PETRONAS, Dept Chem Engn, Bandar Seri Iskandar 32610, Perak, Malaysia
[2] Univ Teknol PETRONAS, Ctr Res Ion Liquids CORIL, Bandar Seri Iskandar 32610, Perak, Malaysia
来源：
PROCEEDING OF 4TH INTERNATIONAL CONFERENCE ON PROCESS ENGINEERING AND ADVANCED MATERIALS (ICPEAM 2016) | 2016年 / 148卷
关键词：
Biosurfactant; rhamnolipid; Pseudomonas aeruginosa; ammonium sulphate precipitation; acid precipitation; zinc sulpahate precipitation; solvent extration; BIOSURFACTANT PRODUCTION;
D O I：
10.1016/j.proeng.2016.06.538
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The high cost of downstream separation, isolation and purification of rhamnolipids are significantly high and this hampers its widespread utilization. Hence, practical and economically sound recovery methods are often sought after. This work focuses on assessing various recovery methods of rhamnolipid produced by Pseudomonas aeruginosa. These methods include that of; acid precipitation, ammonium sulphate precipitation, zinc sulphate precipitation and solvent extraction. Among these methods organic solvent extraction was found to be the best recovery technique giving the highest yield (7.5 g/L) of biosurfactant, while acid precipitation yielded the least at 3.5 g/L. Biosurfactant was supplemented with 1% crude oil (TAPIS) as the carbon source. Emulsification index (E-24) of rhamnolipids to TAPIS was 42%. (C) 2016 The Authors. Published by Elsevier Ltd.
引用
收藏
页码：494 / 500
页数：7
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