Pyrimethamine-loaded lipid-core nanocapsules to improve drug efficacy for the treatment of toxoplasmosis

被引：16
|
作者：
Pissinate, Kenia [1 ,4 ]
Martins-Duarte, Erica dos Santos [1 ,2 ]
Schaffazick, Scheila Rezende [1 ,3 ]
de Oliveira, Catiuscia Padilha [3 ]
Vommaro, Rossiane Claudia [1 ,2 ]
Guterres, Silvia Staniscuaski [3 ]
Pohlmann, Adriana Raffin [3 ,4 ]
de Souza, Wanderley [1 ,2 ,5 ]
机构：
[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio De Janeiro, Brazil
[2] Univ Fed Rio Grande do Sul, Inst Nacl Ciencia & Tecnol Biol Estrutural & Bioi, BR-91501970 Porto Alegre, RS, Brazil
[3] Univ Fed Rio Grande do Sul, Fac Farm, Programa Posgrad Ciencias Farmaceut, BR-91501970 Porto Alegre, RS, Brazil
[4] Univ Fed Rio Grande do Sul, Inst Quim, Dept Quim Organ, BR-91501970 Porto Alegre, RS, Brazil
[5] Inst Nacl Metrol & Qualidade Ind Inmetro, Rio De Janeiro, Brazil
来源：
PARASITOLOGY RESEARCH | 2014年 / 113卷 / 02期
关键词：
DIHYDROFOLATE-REDUCTASE; NANOMEDICINES; CHALLENGES; INHIBITORS; SIZE;
D O I：
10.1007/s00436-013-3715-6
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
We propose an innovative product based on the nanoencapsulation of pyrimethamine (PYR), aiming an improvement of drug efficacy for the treatment of toxoplasmosis. The in vitro cytotoxicity effect of encapsulated PYR and PYR-colloidal suspension was concomitantly evaluated against LLC-MK2 lineage and mouse peritoneal macrophage showing that the cells had similar tolerance for both PYR encapsulated or in the aqueous suspension. CF1 mice acutely infected with tachyzoites of Toxoplasma gondii RH strain treated with different doses (5.0-10 mg/kg/day) of PYR-nanocapsules had survival rate higher than the animals treated with the same doses of non-encapsulated PYR. Thus, encapsulation of PYR improved the efficacy of this drug against an acute model of toxoplasmosis in mice and can be considered an alternative for reducing the dose of PYR, which, in turn, could also reduce the side effects associated to the treatment.
引用
收藏
页码：555 / 564
页数：10
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