Isolation, Purification, and Structural Identification of an Antifungal Compound from a Trichoderma Strain

被引:14
|
作者
Li, Chong-Wei [1 ,2 ]
Song, Rui-Qing [1 ]
Yang, Li-Bin [1 ,3 ]
Deng, Xun [4 ]
机构
[1] Northeast Forestry Univ, Coll Forestry, Harbin 150040, Peoples R China
[2] Heilongjiang Univ, Coll Life Sci, Key Lab Microbiol, Harbin 150080, Peoples R China
[3] Heilongjiang Acad Land Reclamat Sci, Harbin 150038, Peoples R China
[4] Heilongjiang Forestry Acad, Forestry Protect Inst, Harbin 150040, Peoples R China
关键词
Trichoderma; antifungal; biocontrol; bioactive component; structural identification; Cytospora chrysosperma; HARZIANUM;
D O I
10.4014/jmb.1410.10027
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Trichoderma strain T-33 has been demonstrated to have inhibitory effect on the fungus species Cytospora chrysosperma. Here, an active antifungal compound was obtained from Trichoderma strain T-33 extract via combined separation technologies, including organic solvent extraction, liquid chromatography, and thin-layer chromatography. The purified compound was further characterized by advanced analytical technologies to elucidate its chemical structure. Results indicated that the active antifungal compound in Trichoderma strain T-33 extract is 2,5-cyclohexadiene-1,4-dione-2,6-bis (1,1-dimethylethyl).
引用
收藏
页码:1257 / 1264
页数:8
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