The Emergence of Theileria parva in Jonglei State, South Sudan: Confirmation Using Molecular and Serological Diagnostic Tools

被引：7

作者：

Marcellino, W. L. ^{[1
,2
]}

Salih, D. A. ^{[1
,3
]}

Njahira, M. N. ^{[1
]}

Ndiwa, N. ^{[4
]}

Araba, A. ^{[2
]}

El Hussein, A. M. ^{[3
]}

Seitzer, U. ^{[5
]}

Ahmed, J. S. ^{[5
]}

Bishop, R. P. ^{[4
]}

Skilton, R. A. ^{[1
,6
]}

机构：

[1] Biosci Eastern & Cent Africa Int Livestock Res In, Nairobi, Kenya

[2] Minist Anim Resources & Fisheries, Juba, South Sudan

[3] Vet Res Inst, Khartoum, Sudan

[4] ILRI, Nairobi, Kenya

[5] Res Ctr Borstel, Div Vet Infect Biol & Immunol, Borstel, Schleswig Holst, Germany

[6] Int Ctr Insect Physiol & Ecol, POB 30772, Nairobi, Kenya

来源：

TRANSBOUNDARY AND EMERGING DISEASES | 2017年 / 64卷 / 04期

基金：

比尔及梅琳达.盖茨基金会;

关键词：

East Coast fever; emerging disease; Theileria parva; Rhipicephalus appendiculatus; South Sudan; CENTRAL EQUATORIA STATE; EAST-COAST FEVER; TICK-BORNE DISEASES; CARRIER STATE; CATTLE; SEROPREVALENCE; INFECTION; IMPACT;

D O I：

10.1111/tbed.12495

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A cross-sectional survey was carried out in four counties of Jonglei State, South Sudan, between May and June 2012 to determine the distribution and northern limit of Theileria parva, the causative agent of East Coast fever in cattle, and its tick vector Rhipicephalus appendiculatus, as a prerequisite to the deployment of relevant control strategies. A total of 1636 ticks, 386 serum samples and 399 blood samples were collected from indigenous, apparently healthy, cattle of different agegroups. Tick species were identified morphologically, and the identity of R.appendiculatus was confirmed by DNA barcoding. Overall, the T.parva infection rate in R.appendiculatus was 25% as shown by nested PCR. ELISA was used to assess antibodies to T.parva, and the overall seroprevalence was 22.8%. PCR of the blood samples showed 55 (13.8%) were positive for T.parva. This is the first molecular confirmation of T.parva DNA in areas north of Juba, where it was previously known and established. The northern limit of T.parva was determined as N degrees 06.17.792, about 242Km north from Juba. Implication of this limit on the epidemiology and control of ECF is discussed.

引用

页码：1229 / 1235

页数：7

共 4 条

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