A Microflow Cytometer Based on a Disposable Microfluidic Chip With Side Scatter and Fluorescence Detection Capability

被引：13
|
作者：
Xun, Wenpeng [1 ]
Feng, Jianguo [1 ]
Chang, Honglong [1 ]
机构：
[1] Northwestern Polytech Univ, Minist Educ, Key Lab Micro & Nano Syst Aerosp, Xian 710072, Peoples R China
来源：
IEEE TRANSACTIONS ON NANOBIOSCIENCE | 2015年 / 14卷 / 08期
关键词：
Flow cytometer; fluorescence; microfluidics; polydimethylsiloxane; side scatter; FLOW-CYTOMETRY; PARTICLES; LIGHT;
D O I：
10.1109/TNB.2015.2455073
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this paper, we present a microflow cytometer based on a polydimethylsiloxane (PDMS) microfluidic chip. The PDMS chip has a smooth sidewall because of the proposed double molding process. Thus, the side scatter (SSC) detection can be used for the cytometer. The coincidence error using the sheathless focus mechanism is less than 0.069%. Using Flow-Check alignment beads for the counting experiment, the coefficient of variation (CV) of the SSC and the fluorescein isothiocyanate (FITC) channel can reach 8.37% and 2.46%, respectively. The gating of the lymphocytes according to CD45/SSC was also demonstrated successfully. It proves that the chip is a promising solution for point of care (POC) microflow cytometers.
引用
收藏
页码：850 / 856
页数：7
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