Mass spectral profiling of caseinomacropeptide extracted from feeding material and jejunal fluid using three methods-ethanol precipitation, perchloric acid precipitation, and ultrafiltration

被引：9

作者：

Koh, Jeewon ^{[1
]}

Kim, Bum Jin ^{[1
]}

Qu, Yunyao ^{[1
,2
]}

Dallas, David C. ^{[1
,2
]}

机构：

[1] Oregon State Univ, Coll Publ Hlth & Human Sci, Sch Biol & Populat Hlth Sci, Nutr Program, 2520 SW Campus Way, Corvallis, OR 97331 USA

[2] Oregon State Univ, Dept Food Sci & Technol, 100 Wiegand Hall, Corvallis, OR 97331 USA

来源：

FOOD CHEMISTRY | 2023年 / 398卷

关键词：

Caseinomacropeptide; LC-MS/MS; Jejunal fluid; Ethanol precipitation; Perchloric acid precipitation; Ultrafiltration; BIOACTIVE PEPTIDES; GLYCOMACROPEPTIDE; RELEASE; GUT;

D O I：

10.1016/j.foodchem.2022.133864

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The ability of bovine.-casein-derived caseinomacropeptide (CMP) to exert bioactivity in the human gut depends on its digestive survival. Sampling from the human jejunum after feeding CMP and top-down glycopeptidomics analysis facilitates the determination of CMP survival. To reduce interference from non-target molecules in mass spectrometric analysis, CMP must be isolated from digestive fluid. To identify an optimal extraction method, this study compared the profiles of CMP extracted from feeding material (commercial CMP in water) and digestive fluid by ethanol precipitation, perchloric acid (PCA) precipitation, and ultrafiltration. Ethanol precipitation yielded the highest ion abundances for aglycosylated CMP and glycosylated CMP in both feeding material and jejunal samples. Notably, PCA precipitation yielded the highest abundance of partially digested CMP-derived fragments in jejunal samples. Overall, ethanol precipitation was the most effective among the methods tested for intact CMP extraction from jejunal fluids, whereas PCA precipitation was optimal for extraction of CMP fragments.

引用

页数：8