The colonic metabolism differences of main alkaloids in normal and colitis mice treated with Coptis chinensis Franch. and Sophora flavescens Ait. herbal pair using liquid chromatography-high resolution mass spectrometry method combined with chemometrics

被引：7

作者：

Li, Qin ^{[1
,2
]}

Chen, Zihan ^{[2
,4
]}

Zhang, Jianrong ^{[1
]}

Yan, Qiuying ^{[2
,3
]}

Liu, Li ^{[2
,3
]}

Tang, Dongxin ^{[3
]}

Li, Liu ^{[2
,3
]}

Zhang, Jinge ^{[1
]}

Hu, Cheng ^{[1
]}

Ma, Jiayi ^{[1
]}

Cheng, Haibo ^{[2
,3
]}

Kang, An ^{[1
,2
]}

Sun, Dongdong ^{[1
,2
,3
,4
]}

机构：

[1] Nanjing Univ Chinese Med, Sch Pharm, 138 Xianlin Ave, Nanjing 210023, Peoples R China

[2] Nanjing Univ Chinese Med, Collaborat Innovat Ctr Jiangsu Prov Canc Prevent &, 138 Xianlin Ave, Nanjing 210023, Peoples R China

[3] Nanjing Univ Chinese Med, Res Ctr Pathogenesis Theory Cancerous Toxin & Appl, Nanjing, Peoples R China

[4] Nanjing Univ Chinese Med, Sch Integrated Chinese & Western Med, 138 Xianlin Ave, Nanjing 210023, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2023年 / 46卷 / 14期

基金：

中国国家自然科学基金;

关键词：

colonic metabolism; Coptis chinensis Franch; high-resolution mass spectrometry; Sophora flavescens Ait; ulcerative colitis; INFLAMMATION; BERBERINE; LIVER;

D O I：

10.1002/jssc.202300094

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Coptis chinensis Franch. and Sophora flavescens Ait. is a herbal pair frequently used in treating ulcerative colitis. However, the bio-disposition profile of the major components in the inflamed gut remains unclear, which is essential to understand the pharmacological material basis of this herb pair. Here we established an integral quantitative and chemometric method to deduce the colonic metabolism differences of this herbal pair in normal and colitis mice. With this LC-MS method, a total of 41 components have been found in the Coptis chinensis Franch. and Sophora flavescens Ait. extract, and 28 metabolites were found in the colon after oral administration. Alkaloid and its phase I metabolites were the main components in the colon of normal and colitis mice. The results of principal component analysis at 6 h after oral administration showed significant colonic metabolism differences between normal and colitis mice. Heamap results showed that colitis induced significant changes in the colonic bio-disposition of this herbal pair extract. In particular, in the context of colitis, the phase I metabolism of berberine, coptisine, jatrorrhizine, palmatine,and epiberberine has been inhibited. These results may provide a basis for understanding the pharmacological material basis of Coptis chinensis Franch. and Sophora flavescens Ait. in treating ulcerative colitis.

引用

页数：15