Rapid and sensitive point-of-care PTS-CRISPR assay for food safety monitoring of aflatoxin B1

被引：4

作者：

Deng, Ziqiang ^{[1
]}

Zhou, Jin ^{[2
]}

Wang, Chaoqun ^{[2
]}

Hu, Jianyu ^{[3
]}

Liu, Rui ^{[1
]}

Lv, Yi ^{[2
]}

机构：

[1] Sichuan Univ, Minist Educ, Key Lab Green Chem & Technol, Chengdu 610064, Peoples R China

[2] Sichuan Univ, Analyt & Testing Ctr, Chengdu 610064, Peoples R China

[3] Univ Alberta, Div Analyt & Environm Toxicol, Dept Lab Med & Pathol, Fac Med & Dent, Edmonton, AB T6G 2G3, Canada

来源：

CHEMICAL COMMUNICATIONS | 2023年 / 59卷 / 80期

关键词：

AMPLIFICATION; FLUORESCENCE; BIOSENSOR;

D O I：

10.1039/d3cc03984f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Food safety is crucial for human health, but its effective on-site monitoring remains a challenge. Pregnancy test strip (PTS) is the successful point-of-care testing (POCT) product of the highest market share in the world, with the cost as low as $0.10 per test. Herein, combined with the CRISPR (clustered regularly interspaced short palindromic repeats) system, PTS-CRISPR was for the first time introduced into food safety monitoring, for rapid and sensitive POCT of aflatoxin B1. The low-cost, easy-to-operate PTS-CRISPR is expected to bring security to the grassroots food market. Combined with the CRISPR system, PTS-CRISPR was introduced into food safety monitoring for rapid and sensitive POCT of aflatoxin B1.

引用

页码：12011 / 12014

页数：4

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