Mediator Monomer Regulated Emulsion Interfacial Polymerization to Synthesize Nanofractal Magnetic Particles for Nucleic Acid Separation

被引：2

作者：

Shen, Xinyi ^{[1
,2
]}

Zhang, Yue ^{[1
,2
]}

Wang, Duanda ^{[1
,2
]}

Huang, Yanling ^{[1
]}

Song, Yongyang ^{[1
,2
,3
]}

Wang, Shutao ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Sci, Tech Inst Phys & Chem, CAS Key Lab Bioinspired Mat & Interfacial Sci, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Binzhou Inst Technol, Weiqiao UCAS Sci & Technol Pk, Binzhou 256606, Shandong, Peoples R China

来源：

SMALL METHODS | 2024年 / 8卷 / 04期

基金：

中国国家自然科学基金;

关键词：

emulsion interfacial polymerization; fractal structure; magnetic particles; nucleic acid separation; POROUS AROMATIC FRAMEWORKS; METAL-ORGANIC FRAMEWORKS; SELECTIVE IONIC TRANSPORT; GRAPHENE OXIDE MEMBRANES; MIXED-MATRIX MEMBRANES; CLC CHLORIDE CHANNELS; SUBNANOMETER PORES; PROTON TRANSPORT; K+ SELECTIVITY; INTRINSIC MICROPOROSITY;

D O I：

10.1002/smtd.202300531

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Polymer-based magnetic particles have been widely used for the separation of biological samples including nucleic acids, proteins, virus, and cells. Existing magnetic particles are almost prepared by coating polymers on magnetic nanoparticles (NPs). However, this strategy usually encounters the problem of poor magnetic NPs loading capacity. Here, a series of nanofractal magnetic particles (nanoFMPs) synthesized by a strategy of mediator monomer regulated emulsion interfacial polymerization is presented, which allows effective magnetic NPs loading and show efficient nucleic acid separation performance. The mediator monomers facilitate the dispersion of magnetic NPs in internal phase to achieve higher loading, and the hydrophilic monomers use electrostatic interactions to form surface nanofractal structures with functional groups. Compared with magnetic particles without nanofractal structure, nanoFMPs exhibit a higher nucleic acid extraction capability. This strategy offers an effective and versatile way for the synthesis of nanoFMPs toward efficient separation in various fields from clinical diagnosis to food safety and environmental monitoring.

引用

页数：8