A model for N-to-C direction in prebiotic peptide synthesis

Drawing inspiration from the initiating amino acid modification in biosynthetic peptides, we have successfully demonstrated a biomimetic mechanism for N-to-C terminal extension in prebiotic peptide synthesis. This achievement was accomplished by using acetylated amino acid amides as the N-terminal substrate for peptide synthesis and amino acid amides as the C-terminal extension, with the reaction carried out in a dry-wet cycle at 80 degrees C without requiring any activators. This provides a plausible pathway for the formation of prebiotic peptides. Prebiotic peptide synthesis achieves directional extension from N to C by starting with Ac-AA-NH2 as the N-terminal substrate and using AA-NH2 as the building block of the C-terminal extension.