Analytical sensitivity of a multiplex quantitative PCR for Toxoplasma gondii and Neospora caninum

被引：1
|
作者：
Truong, Marcus [1 ]
Slapeta, Jan [1 ,2 ]
机构：
[1] Univ Sydney, Fac Sci, Sydney Sch Vet Sci, Sydney, NSW 2006, Australia
[2] Univ Sydney, Inst Infect Dis, Sydney, NSW 2006, Australia
来源：
PARASITOLOGY RESEARCH | 2023年 / 122卷 / 04期
关键词：
Cyst-forming coccidia; Real-time PCR; Diagnostics; DNA; Neosporosis; Toxoplasmosis; Hammondia; HAMMONDIA-HEYDORNI; QPCR; DNA; DIAGNOSIS; OOCYSTS; SAMPLES; DOG;
D O I：
10.1007/s00436-023-07796-5
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Cyst-forming coccidia, Toxoplasma gondii and Neospora caninum, are recognised as important causes of animal disease. Molecular diagnostics based on the presence of DNA in animal tissue are required to specifically detect T. gondii and N. caninum while achieving high levels of analytical sensitivity. We optimised available single-plex probe base qPCR assays into a multiplexed qPCR panel to detect cyst-forming coccidia, i.e. T. gondii and N. caninum. The T. gondii assay is based on a 529-bp repetitive (REP) element and the N. caninum assay on the NC5 repetitive region. Using target sequence synthetic DNA, the limit of detection (LOD) was determined to be 100 copies, that is less than a single tachyzoite of either T. gondii or N. caninum. The T. gondii and N. caninum multiplexed qPCR assay optimised in this study can be used to effectively detect parasite DNA for diagnostic purposes in animal tissue.
引用
收藏
页码：1043 / 1047
页数：5
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