The role of EII complex in the bacterial responses to the glucose-survey in clinical Klebsiella pneumoniae isolates

被引:3
|
作者
Horng, Yu-Tze [1 ]
Panjaitan, Novaria Sari Dewi [1 ,2 ]
Tsai, Yi-Jhen [1 ]
Su, Pin-Wei [1 ]
Yang, Hung-Chi [3 ]
Soo, Po-Chi [1 ]
机构
[1] Tzu Chi Univ, Coll Med, Dept Lab Med & Biotechnol, Hualien, Taiwan
[2] Natl Res & Innovat Agcy BRIN, Res Org Hlth, Ctr Biomed Res, Cibinong Sci Ctr, Bogor, West Java, Indonesia
[3] Yuanpei Univ Med Technol, Dept Med Lab Sci & Biotechnol, Hsinchu, Taiwan
来源
PLOS ONE | 2023年 / 18卷 / 08期
关键词
PROTEIN-PHOSPHORYLATION; PHOSPHOTRANSFERASE;
D O I
10.1371/journal.pone.0289759
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Type 3 fimbriae in Klebsiella pneumoniae are important for bacterial colonization on abiotic and biotic surfaces. The major subunit of type 3 fimbriae (MrkA) is increased by overexpression of EtcABC, an EII complex of phosphoenolpyruvate:carbohydrate phosphotransferase systems (PTSs), through cAMP-cAMP receptor protein (cAMP-CRP) in K. pneumoniae STU1. Here, we further characterized the relations between the amount of etcABC mRNA and MrkA in 78 clinical K. pneumoniae isolates incubated in high levels of glucose. By Western blotting, we observed that MrkA of 29 isolates were not decreased much by high levels of glucose (Group A) but MrkA of other 49 isolates were significantly reduced (Group B) in the same condition. The bacterial biofilms on abiotic surfaces and colonization in the Caenorhabditis elegans of representative isolates in the Group A were not affected by high levels of glucose. However, the biofilm and colonization in the worm of clinical isolates in the Group B were much reduced by high levels of glucose. After quantification by real time RT-PCR, 76% of Group A but just 10% of Group B showed high amount of etcA mRNA. In summary, our results suggested that for most of K. pneumoniae clinical isolates, the amount of etcABC mRNA was positively related to their type 3 fimbriae production in a high level of glucose, thereby to their biofilm formation and colonization in the worm.
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页数:13
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