Flavobacterium lacisediminis sp. nov., a bacterium isolated from lake sediment

A taxonomic identification using polyphasic approach was performed on strain TH16- 21T, which was isolated from the inter-facial sediment of Taihu Lake, PR China. Strain TH16- 21T was Gram- stain-negative, aerobic, rod-shaped and catalase-positive. Phylogenetic analysis based on the 16S rRNA gene and genomic sequences indicated that strain TH16-21T was classified within the genus of Flavobacterium. The 16S rRNA gene sequence of strain TH16- 21T showed the highest similarity to Flavobacterium cheniae NJ-26T (98.9 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain TH16- 21T and F. cheniae NJ-26T were 91.2 and 45.9 %, respectively. The respiratory quinone was menaquinone 6. The major cellular fatty acids (>10 %) comprised iso-C15:0, iso-C16:0, iso-C15: 1 G and iso- C16: 0 3 -OH. The genomic DNA G+C content was 32.2 mol%. Phos-phatidylethanolamine, six amino lipids and three phospholipids were the main polar lipids. Based on the phenotypic features and phylogenetic position, a novel species with the name Flavobacterium lacisediminis sp. nov. is proposed. The type strain is TH16-21T (=MCCC 1K04592T=KACC 22896T).