Fluorochrome-labeled inhibitors of caspase-1 require membrane permeabilization to efficiently access caspase-1 in macrophages

被引：1

作者：

Thygesen, Sara J. ^{[1
,6
]}

Burgener, Sabrina S. ^{[2
,3
]}

Mudai, Prerna ^{[1
]}

Monteleone, Mercedes ^{[2
,3
]}

Boucher, Dave ^{[5
]}

Sagulenko, Vitaliya ^{[1
]}

Schroder, Kate ^{[2
,3
,4
]}

Stacey, Katryn J. ^{[1
,4
,6
]}

机构：

[1] Univ Queensland, Sch Chem & Mol Biosci, St Lucia, Australia

[2] Univ Queensland, Inst Mol Biosci IMB, St Lucia, Australia

[3] Univ Queensland, IMB Ctr Inflammat & Dis Res, St Lucia, Australia

[4] Univ Queensland, Australian Infect Dis Res Ctr, St Lucia, Australia

[5] Univ York, York Biomed Res Inst, Dept Biol, York, England

[6] Univ Queensland, Sch Chem & Mol Biosci, St Lucia, Qld 4072, Australia

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 2024年 / 54卷 / 05期

基金：

澳大利亚研究理事会; 英国医学研究理事会;

关键词：

Caspase inhibitor; Caspase-1; FLICA; Gasdermin D; Inflammasome; IN-SITU; MECHANISM; ACTIVATION; CLEAVAGE;

D O I：

10.1002/eji.202350515

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores. image

引用

页数：5

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