Effect of acetic acid inactivation of SARS-CoV-2

被引:9
|
作者
Amruta, Narayanappa [1 ]
Maness, Nicholas J. [2 ,3 ]
Gressett, Timothy E. [1 ,4 ]
Tsuchiya, Yoshihiro [5 ]
Kishi, Mikiya [5 ]
Bix, Gregory [1 ,4 ,6 ]
机构
[1] Tulane Univ, Clin Neurosci Res Ctr, Dept Neurosurg, Sch Med, New Orleans, LA 70118 USA
[2] Tulane Univ, Tulane Natl Primate Res Ctr, Covington, LA USA
[3] Tulane Univ, Dept Microbiol & Immunol, Sch Med, New Orleans, LA USA
[4] Tulane Univ, Tulane Brain Inst, New Orleans, LA 70118 USA
[5] Mizkan Holdings Co Ltd Aichi, Cent Res Inst, Handa, Japan
[6] Tulane Univ, Dept Neurol, Sch Med, New Orleans, LA 70118 USA
来源
PLOS ONE | 2023年 / 18卷 / 02期
关键词
ELECTRON-MICROSCOPY; STABILITY;
D O I
10.1371/journal.pone.0276578
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Effective measures are needed to prevent the spread and infectivity of SARS-CoV-2 that causes COVID-19. Chemical inactivation may help to prevent the spread and transmission of this and other viruses. Hence, we tested the SARS-CoV-2 antiviral activity of acetic acid, the main component of vinegar, in vitro. Inactivation and binding assays suggest that acetic acid is virucidal. We found that 6% acetic acid, a concentration typically found in white distilled vinegar, effectively inactivated SARS-CoV-2 after 15-min incubation with a complete loss of replication of competent virus as measured by TCID50. Transmission electron microscopy further demonstrated that 6% acetic acid disrupts SARS-CoV-2 virion structure. In addition, 6% acetic acid significantly inhibits and disrupts the binding of SARS-CoV-2 spike protein binding to ACE2, the primary SARS-CoV-2 cell receptor, after contact with spike protein for 5, 10, 30 and 60 minutes incubation. Taken together, our findings demonstrate that acetic acid possesses inactivating activity against SARS-CoV-2 and may represent a safe alternative to commonly used chemical disinfectants to effectively control the spread of SARS-CoV-2.
引用
收藏
页数:10
相关论文
共 50 条
  • [1] Inactivation of SARS-CoV-2 Laboratory Specimens
    Haddock, Elaine
    Feldmann, Friederike
    Shupert, W. Lesley
    Feldmann, Heinz
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2021, 104 (06): : 2195 - 2198
  • [2] PMMA Microcapsules for the Inactivation of SARS-CoV-2
    Sousa, Vania I. .
    Parente, Joana F.
    Marques, Juliana F.
    Calcada, Carla
    Veiga, Maria I.
    Osorio, Nuno S.
    Tavares, Carlos J.
    ACS OMEGA, 2022, : 22383 - 22393
  • [3] Assessment of inactivation procedures for SARS-CoV-2
    Auerswald, Heidi
    Yann, Sokhoun
    Dul, Sokha
    In, Saraden
    Dussart, Philippe
    Martin, Nicholas J.
    Karlsson, Erik A.
    Garcia-Rivera, Jose A.
    JOURNAL OF GENERAL VIROLOGY, 2021, 102 (03):
  • [4] Inactivation of SARS-CoV-2 by gamma irradiation
    Jain, Rajlaxmi
    Sarkale, Prasad
    Mali, Deepak
    Shete, Anita M.
    Patil, Deepak Y.
    Majumdar, Triparna
    Suryawanshi, Annasaheb
    Patil, Savita
    Mohandas, Sreelekshmy
    Yadav, Pragya D.
    INDIAN JOURNAL OF MEDICAL RESEARCH, 2021, 153 (1-2) : 196 - 198
  • [5] Inactivation of SARS-CoV-2 in Water by Chlorination
    Samendra Sherchan
    Luisa A. Ikner
    Charles P. Gerba
    Food and Environmental Virology, 2023, 15 : 262 - 264
  • [6] Inactivation of SARS-CoV-2 by Ozonated Glycerol
    Takeda, Yohei
    Jamsransuren, Dulamjav
    Makita, Yoshimasa
    Kaneko, Akihiro
    Matsuda, Sachiko
    Ogawa, Haruko
    Oh, Hourei
    FOOD AND ENVIRONMENTAL VIROLOGY, 2021, 13 (03) : 316 - 321
  • [7] Inactivation of SARS-CoV-2 in Water by Chlorination
    Sherchan, Samendra
    Ikner, Luisa A.
    Gerba, Charles P.
    FOOD AND ENVIRONMENTAL VIROLOGY, 2023, 15 (03) : 262 - 264
  • [8] Inactivation of SARS-CoV-2 by Ozonated Glycerol
    Yohei Takeda
    Dulamjav Jamsransuren
    Yoshimasa Makita
    Akihiro Kaneko
    Sachiko Matsuda
    Haruko Ogawa
    Hourei Oh
    Food and Environmental Virology, 2021, 13 : 316 - 321
  • [9] Effect of Inactivation Methods on SARS-CoV-2 Virion Protein and Structure
    Loveday, Emma K.
    Hain, Kyle S.
    Kochetkova, Irina
    Hedges, Jodi F.
    Robison, Amanda
    Snyder, Deann T.
    Brumfield, Susan K.
    Young, Mark J.
    Jutila, Mark A.
    Chang, Connie B.
    Taylor, Matthew P.
    VIRUSES-BASEL, 2021, 13 (04):
  • [10] Rapid Inactivation of SARS-CoV-2 with Ozonated Water
    Inagaki, Hiroko
    Saito, Akatsuki
    Sudaryatma, Putu Eka
    Sugiyama, Hironobu
    Okabayashi, Tamaki
    Fujimoto, Shouichi
    OZONE-SCIENCE & ENGINEERING, 2021, 43 (03) : 208 - 212