Effects of Hyperbaric Oxygen on Anti-dsDNA Antibodies and IL-6 in BALB/c Mice with Pristane-Induced Lupus Nephritis

Objective: Lupus nephritis (LN) has negative outcomes in patients. It is associated with enhanced oxidative stress. The aim in this study was to explore whether hyperbaric oxygen (HBO) made beneficial effects on the levels of anti-double stranded DNA (dsDNA) antibodies and interleukin-6 (IL-6) within the serum of LN mode treated with HBO compared to LN mode without HBO and negative controls. Materials and Methods: The samples were separated into 3 groups (negative control, LN and LN + HBO groups) in 24 mice. Pristane was injected into female BALB/c mice to make the LN model. After 60 days, HBO therapy was administered to the LN model for 10 sessions. Levels of anti-dsDNA antibodies and IL-6 serum of all groups were examined by ELISA. Results: The level of anti-dsDNA antibodies and IL-6, as the biomarker of LN activity, was essentially diminished within HBO [a statistically significant decrease (p<0.050) between the LN + HBO group and LN group]. Conclusion: The inflammation in LN can be reduced by decreasing anti-dsDNA antibodies and IL-6 levels with HBO through the repairment of hypoxia.