Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysis

被引：2

作者：

Bafor, Enitome E. ^{[1
]}

Martin, Toni ^{[1
]}

Carrell, Jeffrey ^{[2
]}

Karwan, Megan ^{[1
]}

Kimmel, Adrienne E. ^{[1
]}

Omogiade, Uyi. G. ^{[1
]}

Sanford, Michael ^{[1
]}

Young, Howard A. ^{[1
]}

Valencia, Julio C. ^{[1
]}

机构：

[1] NCI, Canc Innovat Lab, Ctr Canc Res, Frederick, MD 21702 USA

[2] Frederick Natl Lab Canc Res, Basic Sci Program, Frederick, MD 21702 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

关键词：

ESTROUS-CYCLE; RAT; PROGESTERONE;

D O I：

10.1016/j.xpro.2023.102710

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we present a validated workflow to isolate sufficient viable single ovary cells from a single mouse without the need to pool from several mice. We provide steps essential for estrous staging, ovary harvesting and dissociation, ovary cell staining, data collection, and analysis. Our approach allows the use of these sin-gle-cell suspensions for flow sorting, flow cytometry analysis, or functional in vitro assays. Importantly, our protocol is designed to maximize the isolation of immune cells, including T cell subsets.

引用

页数：17

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