Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system

被引：0
|
作者：
Ding, Tongbin [1 ]
Gao, Zhe [1 ]
Liu, Hang [1 ]
Li, Yuan [1 ]
Liu, Shichao [1 ]
Jian, Liguo [1 ,2 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp 2, Dept Cardiol, Zhengzhou 450014, Peoples R China
[2] Zhengzhou Univ, Affiliated Hosp 2, Dept Cardiol, 2 Jingba Rd, Zhengzhou 450014, Henan, Peoples R China
来源：
STEM CELL RESEARCH | 2023年 / 67卷
关键词：
D O I：
10.1016/j.scr.2023.103029
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Protein phosphatase 2A (PPP2CA) is one of the four main Ser/Thr phosphatase enzymes, which involved in the negative control of cell growth and division. PPP2CA is the main protein phosphatase in the heart, which regulates Ca (2+) through a series of ion channels and transporters. In this study, we generated a PPP2CA homozygous knockout human embryonic stem cell line WAe009-A-25 based on the transient expression CRISPR/Cas9 system to investigate functional effect of PP1 deficiency. This cell line has multidirectional differentiation potential, normal karyotypic and trilineage differentiation potential in vivo.
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页数：5
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